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Extra resources for ADP-Ribosylation Reactions. Biology and Medicine
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The recycling of the pyridine ring is essentially perfect in exponentially growing E. , 1976a). By comparison with the pyridine ring, other parts of the NAD molecule have a much lower probability of returning to the pyridine nucleotide pool once an NAD molecule is broken down. To measure turnover, cells are grown in radioactive medium, so that both the pyridine ring and the adenine moiety are labeled, but with two distinguishable isotopes. The cells are shifted to an identical unlabeled medium.
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