By Stephen E. Harding, Michael P. Tombs, Gary G. Adams, Berit Smestad Paulsen, Kari Tvete Inngjerdingen, Hilde Barsett
Polysaccharides and similar excessive molecular weight glycans are highly diversified with huge program in Biotechnology and nice possibilities for additional exploitation. An advent to Polysaccharide Biotechnology – a moment variation of the preferred unique textual content through Tombs and Harding – introduces scholars, researchers, clinicians and industrialists to the homes of a few of the main fabrics concerned, how those are utilized, a number of the financial elements relating their construction and the way they're characterised for regulatory purposes.
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Additional info for An Introduction to Polysaccharide Biotechnology
These can provide simultaneously the intrinsic viscosity [η](Ve) and weight-average molecular weight Mw(Ve) both as a function of elution volume Ve. Another useful parameter for determining the overall conformation of a polysaccharide is the ‘Wales–van Holde’ ratio k s/[η], where k s (mL g−1) is the concentration dependence or ‘Gralen’ parameter of the sedimentation coefficient. 6). b. 12 Conformation zones of polysaccharides. A: rigid rod; B: rod with limited flexibility; C: semi-flexible coil; D: random coil; E: globular or highly branched.
When nothing was known about enzymes there was no ambiguity, but now that we know enzymes are often present but with a mutation that makes them inactive, or maybe in an inactive conformation, a more precise definition is needed. In this book, by the ‘enzyme’ we mean the peptide chain specified by the gene, including mutants of the gene, whether or not it is in the active conformation. This has gradually become the commoner meaning of the word, which makes it necessary to specify the activity as a separate point.
It is popularly measured using a thermostatically controlled rotational viscometer, which permits extrapolation to zero shear (in cases where the shear dependence is negligible, simple capillary viscometers can be used). Differential pressure viscometers, which work on a different principle (and with negligible shear effects) can operate at low concentration and can be incorporated online to SEC or SEC–MALS or MALS devices, are becoming increasingly popular. These can provide simultaneously the intrinsic viscosity [η](Ve) and weight-average molecular weight Mw(Ve) both as a function of elution volume Ve.