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The specificity of this reagent for nuclear structures in bacteria was questioned (Bisset, 1953). Further studies are indicated to sustain such criticism, although it seems likely that the technique is a valid one. , 1955). It is claimed that free aldehydes in. the cell membrane and DNA are stained. 100 J so - so - X Emox. 40 - wAVELENOTH IN Mu. 6. Absorption curves of solutions of acid fuchsin (-) and formaldehyde-reacted acid fuchsin-SOz (-----). The curve is shifted to a higher wavelength, just as with the conventional Schiff’s reagent.

Table VI lists some compounds which fail to give a color reaction with Schiffs reagent. Various oxidizing agents, such as chlorates, peroxides, and atmospheric 30 FREDERICK H. , 1933; Chu, 1950 Schmidt, 1881; Bitto, 1897; Lison, 1932 Bitto, 1897 Schmidt, 1881; Bitto, 1897; Lison, 1932 Bitto, 1897 Chu, 1950 Bitto, 1897 Bitto, 1897 Bitto, 1897 Bitto, 1897 Bitto, 1897 Organic bases Antipyrine Pyrrol Lison, 1932 Lison, 1932 Phenols Cinnamylguaiacol Guaiacol Hydroquinone Orcin Phenol Pyrocatechol Pyrogallol Quinone Resorcin Lison, 1932 Schmidt, 1881 Schmidt, 1881 Schmidt, 1881 Schmidt, 1881 Schmidt, 1881 Schmidt, 1881 Schmidt, 1881 Schmidt, 1881 Polysaccharides Inulin Potato starch Tobie, 1942 Josephson, 1923 Sugar alcohols D-Mannitol D-sorbitot Dulcitol Meso-inositol hydrate Tobie, Tobie, Tobie, Tobie, Sugars Glucose Invert sugar Lactose 1942 1942 1942 1942 Schmidt, 1881 ; Bitto, 1897 ; Josephson, 1923 ; Overend, 1950 Bitto, 1897 Schmidt, 1881 32 FREDERICK H.

40 - wAVELENOTH IN Mu. 6. Absorption curves of solutions of acid fuchsin (-) and formaldehyde-reacted acid fuchsin-SOz (-----). The curve is shifted to a higher wavelength, just as with the conventional Schiff’s reagent. Data from Kasten (19%). Unaware initially of the reports cited above, Kasten (1956a, 1958a, 1959) made an extensive survey of dyes to find new Schiff-type reagents, to e m o n s t r a t e by standard cytochemical method? the aldehyde specificity of such reagents, to utilize these colorimetric reagents in multiple-staining techniques, and to use such reagents to study cytochemical polyaldehyde reaction mechanisms.

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