By Alain Berthod
Micellar Liquid Chromatography experiences using surfactant recommendations at or above the serious micelle focus as cellular stages in liquid chromatography. It employs a computer-assisted optimization technique and integrates micellar liquid chromatography (MLC) with different chromatographic and electrophoretic concepts utilizing surfactants. it's also the MICHROM software program package deal on CD-ROM to facilitate the applying of equations and optimize potency of MLC platforms.
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Additional info for Micellar Liquid Chromatography (Chromatographic Science, V. 83.)
Of sulhric acid ester form the alkyl sulfate surfactant family. They are produced by sulfation with sulfur trioxide of linear alcohols: The al~lsulfuricacid formed is a strong acid, easilyneutralized by s o d i ~ or potassium hydroxide. The alkylsulfate aqueous solutionsare neutral and sis occurs in veryacidic solutions. O,Na is the most c o ~ o member n S physicochemical properties have been e~tensively investigated [3,4]. AZ ~ ~ ~ hisfamily ~ includes s ~ alkylesters of phosp d The alkylphosphates are low in aqueous solutions, even at extreme ~ the ~salts ~of thee mono ~i- .
The amine oxides, such as the alkyl dimethyl amineoxides: are no~onic su~actants in basic and neutral solutions. They are protonated in acidic solutions and, thus, represent the transition to cationic su~actants. Amphoteric su~actantsare ionic sudactants containing positive and negative charges on the same molecule . They can be true amphoteric ions, such as the betaines: with n r 1, that have a cationic nature in strongly acidic media and an amphoteric structure in neutral and basic solutions.
Evaluate the solute and Vmvolumes are us ses, the solute micellar p m coe~cient, also call micellar ~ i ~ ~co~stant, ~ i and ~ ~ the ~ solute o local n concentr micellar or aqueous phase. e micelle size depends onthe aggregation number. cture of micelles should be kept in mind. 7. ~ZceZZesare, in fact, an a~imateddist true single monomers;4 . 7). 1 M SDS solution at 20°C. The SDS micelle aggregationnumber varies from 50 to 80 with an average value of 62. The micelle shape dependson thesurfactant concentration.