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By J.N. Davidson, Waldo E. Cohn (Eds.)

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68,997 (1961). 91. S. Bresler, V. Kalinin and D. A. 6, 1 (1968). 92. E. P. Geiduschek, Proc. Xatl. Sci. 47, 950 (1961). 93. E. F. , B. K. Zimmerman, and E. P. Bid. 8, 377 85. 86. 87. 88. 89. (1964). 94. E. Freese and H. Null. 48, 1796 (1962). 95. E. Bautz-Freese, J. Gerson, H. -J. Rhaese and E. 4, 517 (1967). E. Freese and E. B. Freese, Biochemistry 4, 2419 (1965). L. Wierzchowski and D. Shugar, Photochem. Photobiol. 1, 21 (1962). 98. R. B. Setlow, Proc. Xatl. Sci. 63, 1111 (1965). 99. D. B. Ludlum, Biochim.

There remains the question, why methylation was the most mutagenic of all alkylations in RNA (@), while the ethylating agents were generally found to be the most effective mutagens in double-stranded DNA. 26. ROLE OF CONFORMATION I N CHEMICAL MUTAGENESIS 21 riLosyl bond at N-9 is vcry lubile w l i ~ i :iL incthyl group quaternizes the N-7 positioti, but :tccording to ittost :iuthors it is less cffected by an ethyl 101). 2), or to more rapid testing of the alkylated samples. As a consequence of the protecting action of double-strandedness, side reactions play a much greater role in DNA and may account for a great part of the inactivating and mutagenic events obtained with reagents affecting the amino groups.

Gordon, Biochim. Biophys. Acta129, 32 (1966). 69. K. S. Shastry and M. Gordon, Biochim. Biophys. Acta129, 42 (1966). L. A. Waskell, K. S. Shastry, and M. Gordon, Biochim. Biophys. Acta129, 49 (1966). 61. M. I. Simon, L. Grossman, and H. Biol. 12, 50 (1965). P. Chandra and A. Naturforsch. 2lb, 663 (1966). 65. B. Singer and H. Fraenkel-Conrat, Biochemistrp 4, 226 (1965). J. K. 2,197 (1966). R. L. Sinsheimer and R. Hastings, Science 110, 525 (1949). 66. K. L. Wierzchowski and D. Shugar, ActaBiochim.

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