By John N. Abelson, Melvin I. Simon, Alfred H. Merrill Jr., Yusuf A. Hannun
Sphingolipids are present in all eukaryotic and in a few prokaryotic organisms and supply constitution for mobile membranes, lipoproteins, and different organic fabrics in addition to perform the law of mobile development, differentiation, and numerous telephone services, together with cell-cell verbal exchange, cell-substratum interactions, and intracellular sign transduction. This quantity offers equipment utilized in learning enzymes of sphingolipid biosynthesis and turnover, together with inhibitors of a few of those enzymes, genetic techniques, and natural and enzymatic syntheses of sphingolipids and analogs. Its significant other Volume 312 will comprise info on reading sphingolipids, sphingolipid shipping and trafficking, and sphingolipid-protein interactions and mobile targets.
The significantly acclaimed laboratory ordinary for greater than 40 years, Methods in Enzymology is among the so much hugely revered guides within the box of biochemistry. for the reason that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by way of researchers and reviewers alike. Now with greater than three hundred volumes (all of them nonetheless in print), the sequence comprises a lot fabric nonetheless correct today--truly an important book for researchers in all fields of existence sciences
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Additional info for Sphingolipid Metabolism and Cell Signaling Part A
6 mm with a 10-mm guard column). Elution is isocratic with 90% methanol. Absorbance of the effluent is monitored at 280 nm (Fig. 2). Exact elution times will vary with the HPLC apparatus used, but an absorbance peak representing the derivatized enzyme product, biphe9 F. B. Jungalwala, J. E. Evans, E. Bremer, and R. H. McCluer, J. Lipid Res. 24, 1380 (1983). lO R. C. Dickson, G. B. Wells, A. Schmidt, and R. L. Lester, Mol. Cell, Biol. 10, 2176 (1990).  S. cerevisiae DIHYDROSPHINGOSINE 4-HYDROXYLASE 13 solvent front --~ DHS--~ PHS--~ origin--* ii FIG.
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